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51st ERA-EDTA CONGRESS

 

31 May - 3 June 2014 AMSTERDAM
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Discussion forum - 51st ERA-EDTA CONGRESS
    Topic - M) Dialysis. M1) Extracorporeal dialysis: techniques and adequacy.

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Poster: 410
Visits: 28
Title: EVALUATION OF CLEAR D-tectTM BIOLUMINESCENCE METHOD: A NEW CHEMICAL TOOL TO MONITOR IN 10 MINUTES THE MICROBIAL QUALITY OF HEMODIALYSIS WATER
Authors: ALAIN RAGON , E.VANNIER; E.MATTIO; V.TODOROVA; P.BRUNET
Centre: Assistance Publique Hopitaux de Marseille

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EVALUATION OF CLEAR D-TECTTM BIOLUMINESCENCE METHOD: A NEW CHEMICAL TOOL TO MONITOR IN 10 MINUTES THE MICROBIAL QUALITY OF HEMODIALYSIS WATER

ALAIN RAGON
Thread initiator
Subspeciality
Reg: 5/15/2014 6:06:00 PM
Comment# 1
Microbial quality of dialysis water is monitored with 2 indicators: bacterial endotoxins levels and culture of bacteria. Plate counting is the standard method used to estimate the number of viable cells present in the water sample expecting that bacteria can give rise to CFU (Colony Forming Unit) under specific conditions of nutrient medium, temperature and time. Unfortunately there is no culture medium, temperature and incubation time suitable for all bacteria. For these reasons the number of CFU underestimate the microbial contamination. Furthermore an incubation time of 7 days is recommended. Safety management of microbial risk requires a reliable and fast method to detect a microbial contamination of dialysis water. A new kit D-tect based on bioluminescence measurement of ATP (Adenosine TriPhosphate) is an alternative technique to rapidly detect viable bacterial cells in ultrapure water samples.

Comment added on 5/15/2014 6:06:00 PM 
     
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Most viewed poster for this congress
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